Title : Epidemiological survey with antigenic fragments of Hsp83 and kPCR in population at risk for leishmaniasis
Abstract:
Leishmaniasis in Peru is a public health concern and affects inhabitants of endemic areas and also people who go to jungle regions for short or long time, where the Leishmania parasite fulfills its life cycle. There is a high incidence of cutaneous and mucocutaneous leishmaniasis in the country, and infections vary from mild cutaneous lesions to disfiguring mucocutaneous lesions. Mucosal lesions can be expressed in the patient from 1 year to 20 years after the first lesion, so it is important to have a sufficient sensitive and specific technique to detect the disease in its early stages. To carry out the study we used ELISA with antigenic fragment of the Hsp83 protein, which has a high potential for the diagnosis of leishmaniasis and PCR whose target is the DNA of the kinetoplast, with 75pb amplicon. This study aimed to know the scope of each of the tests for the diagnosis of patients with diverse clinical aspects, who live in or have visited endemic regions. The study was done between 2023-2024, 100 biological samples were analyzed using ELISA and kPCR. The epidemiological data was obtained of patient’s survey and we are going to analyze and discuss the potential of the molecular and serological technique, and the use of both of them in accord to the epidemiological and clinic characteristics.
