Title : Performance of gold and selenium nanoparticles in lateral flow assays for HIV p24 detection
Abstract:
Human Immunodeficiency Virus (HIV) remains a significant global health challenge, necessitating the development of effective diagnostic tools for early detection and timely intervention. Among the various biomarkers for HIV detection, the p24 antigen serves as a pivotal marker, especially during the acute phase of infection, where viral replication and contagiousness are high, but seroconversion has not yet occurred. The ability to detect p24 antigen in the early stages of infection offers a crucial opportunity for prompt diagnosis, enabling early treatment strategies. Lateral Flow Assay (LFA) is a widely adopted diagnostic technique due to its simplicity, rapidity, and cost-effectiveness, particularly suitable for point-of-care settings. LFA systems, when tailored for p24 detection, facilitate fast and reliable HIV diagnosis without the need for specialized laboratory infrastructure.
Incorporating nanoparticles into LFA systems has significantly enhanced their sensitivity and detection capabilities, particularly for low-concentration biomarkers such as p24. Gold (Au) and Selenium (Se) nanoparticles are among the most commonly employed materials in LFA applications, owing to their distinct optical properties, ease of functionalization, and stability. Alt?n nanopartiküller (AuNPs), yüksek oranda tespit edilebilir sinyaller sa?layan güçlü optik özellikleri nedeniyle yayg?n olarak kullan?l?rken, selenyum nanopartiküller (SeNPs) benzersiz optik davran?? ve çe?itli çevresel ko?ullarda geli?mi? stabilite sunarak umut verici alternatifler sunmaktad?r.
This study investigates the application of different-sized Gold and Selenium nanoparticles in the LFA-based detection of p24 HIV antigen. By exploring nanoparticle size optimization, the study aims to improve assay sensitivity and specificity. The findings of this work underscore the significant impact of nanoparticle size on the overall performance of the LFA system, demonstrating the potential of both AuNPs and SeNPs as highly efficient labels for p24 detection. The results have important implications for the development of more sensitive, reliable, and accessible LFA-based diagnostic tools for HIV, paving the way for advanced point-of-care diagnostic solutions.
