Title : Staphylococcus aureus isolated from clinical samples and its antibiogram analysis
Abstract:
Staphylococcus aureus is one of the most rapidly stated microbes among the all pathogens that are present in hospitals. In burn unit S. aureus remains a main origin of diseases. S. aureus is also the part of our normal flora but its infection begins when it gets an entry in our body via cut, burns, and wound or through any other source. This study was conducted with an aim to isolate S. aureus from different clinically important samples of the patients and also to find an antimicrobial susceptibility pattern of the mentioned bacteria. Different Microbiological Standards and procedures including identification of colony’s morphology by growing bacteria on different media, gram staining, microscopy and biochemical tests were used for the analysis of clinical specimens taken from blood, pus swab and urine. Out of 399 samples 119 (29.82%) were positive isolates with a high percentage in pus swab (44.01%), the percentage of blood and urine was 16.32% and 11.95% respectively. According to the gender-wise distribution, the percentage in male was (53.78%) and in female was (46.21%). Antibiotic susceptibility procedures were applied in this study to find out the susceptibility as well as the resistivity of S. aureus to various antibiotics. Staphylococcus aureus showed resistance to Penicillins (100%), Ciprofloxacin (78.15%), Clindamycin (58.82%), Amikacin (56.30%), Cefoxitin (21.84%), Gentamicin (52.94%), Chloramphenicol (30.25%), Rifampicin (30.25%), Doxycycline (52.10%), and Minocycline (47.05%), while resistivity toward Vancomycin and Linezolid was 0%. Its sensitivity rate was as follows: Vancomycin (100%), Linezolid (100%), Cefoxitin (78.15%), Chloramphenicol (69.74%), Rifampicin (69.74%), Minocycline (52.94%), vi Ciprofloxacin (21.84%), Clindamycin (41.17%), Gentamicin (47.05%), Amikacin (43.69%), Doxycycline (47.89%), while sensitivity toward Penicillins was 0%.
Keywords: Antibiotic resistance; Antimicrobial susceptibility; Staphylococcus aureus; Gram negative.
