Title : The combination (amphotericin B + EDTA) as an antifungal blocking agent in the inhibition of mixed biofilm formation on catheters
Abstract:
Introduction: Vascular and urinary catheterization are routine procedures in hospitals, exposing patients to the risk of infection, the pathophysiology of which is closely linked to the presence of microorganisms on these implants. The ability to form biofilms is considered an important virulence factor for pathogenic microorganisms. The potential to serve as an infectious reservoir for a variety of bacteria and yeasts, makes biofilms difficult to diagnose and treat, requiring complex poly-chemotherapy treatment strategies.
Objective: The aim of this study was to evaluate the efficacy of the antimicrobial locking technique (ALT) on mixed biofilms (non albicans Candida species/bacteria) formed on medical catheters, using ethylene diamine tetra-acetic acid (EDTA) and amphotericin B (AmB) as antimicrobial agents.
Mixed biofilms of non albicans Candida species/ bacteria, co-isolated from the same medical device, were cultured on catheters and incubated with a locked solution of EDTA and AmB for 6 and 8 hours.
Results: Our results suggest that AmB combined with EDTA could be a useful ALT agent in the treatment of catheter-related infections.
Keywords: mixed biofilms (non albicans Candida species/ bacteria), medical device, lock solution, EDTA and amphotericin B.
