In the domain of Diagnostic Microbiology, the study of antimicrobials holds paramount significance. The accurate and timely identification of microorganisms and their susceptibility to antimicrobial agents is imperative for effective patient management. Diagnostic microbiology employs a range of methodologies, including culture-based techniques, biochemical assays, and molecular diagnostics, to provide comprehensive insights into microbial infections. The integration of rapid diagnostic technologies has revolutionized the field, enabling healthcare professionals to make informed decisions on antimicrobial therapy promptly. Diagnostic microbiologists play a crucial role in combating antibiotic resistance by guiding clinicians towards judicious and targeted use of antimicrobials. In essence, Diagnostic Microbiology stands at the forefront of ensuring precision and efficacy in antimicrobial interventions, thereby contributing substantially to the practice of modern medicine.