Protozoal infections constitute a diverse group of illnesses caused by parasitic protozoa, single-celled microorganisms that can infect various tissues and organs in humans. Malaria, one of the most prevalent protozoal infections, is caused by Plasmodium parasites transmitted through the bites of infected mosquitoes. The symptoms range from fever and chills to severe complications, posing a significant public health challenge, particularly in tropical and subtropical regions. Another notable protozoal infection is Trypanosomiasis, including African sleeping sickness caused by Trypanosoma brucei and Chagas disease caused by Trypanosoma cruzi. These infections often involve complex life cycles, with transmission occurring through vectors such as tsetse flies or reduviid bugs. Amebiasis, caused by the protozoan Entamoeba histolytica, leads to gastrointestinal symptoms and can result in severe complications, including liver abscesses. Protozoal infections vary widely in their clinical manifestations, necessitating accurate diagnosis and targeted treatment approaches to address the diverse challenges posed by these parasitic microorganisms.