Infectious diseases remain a persistent global health challenge, requiring accurate and timely diagnosis for effective management and control. Molecular diagnostics, a rapidly evolving field at the intersection of biology and technology, offers powerful tools for the detection and characterization of infectious agents at the molecular level. By analyzing nucleic acids or proteins specific to pathogens, molecular diagnostic tests provide rapid and precise identification of infectious diseases, often surpassing the capabilities of traditional culture-based methods. Techniques such as polymerase chain reaction (PCR), nucleic acid sequencing, and immunoassays enable clinicians to detect a wide range of pathogens with high sensitivity and specificity, facilitating early intervention and targeted treatment strategies. Additionally, molecular diagnostics play a crucial role in monitoring antimicrobial resistance patterns, identifying emerging pathogens, and guiding public health interventions to prevent outbreaks and mitigate the spread of infectious diseases.